The tire and wheel are going in today for inspection and replacement of the tube and, I have decided to replace the tire as well (current tire has 60-70% tread left but will be kept as a spare), will run through all of the posted suggestions at the same time.
I will discuss installing a rim lock as well, however, my rim/tire mark does not indicate that the tire has moved on the rim. As stated earlier, I run my rear at 28-30 psi and most riders that I have spoken to are running at 25 psi with-out issues.
I removed the rear wheel last night and cannot find any indication of a puncture any where. Will post again once tire has been removed and inspected, and report any discoveries.
To all: Thanks for the replies and suggestions.